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Using the information obtained from the enzyme animation in the virtual lab and your textbook, answer the following questions in the lab report.
Which of the following does NOT apply to an enzyme?
Select one:
a. Catalyst
b. Inorganic
c. Protein
d. All of the above apply to an enzyme
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Which of the following would interfere most with the ability of an enzyme to catalyze a reaction?
Select one:
a. Reduced concentration of substrate available
b. Reduced concentration of product available
c. Increased concentration of substrate available
d. A change in the pH
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Feedback mechanisms regulate the rate of enzyme activity, effectively “turning off” an enzyme in a reversible way until more product is needed. Which of the following would be most effective as a feedback mechanism?
Select one:
a. Reduced concentration of product
b. Increased concentration of substrate
c. A change in pH
d. Temporary binding of a non-substrate molecule in the active site
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Which of the following statements is accurate in describing the activity of the lactase enzyme?
Select one:
a. Lactase can function equally effectively at many different pH levels
b. The shape of lactase does not change during the reaction
c. Lactase is converted to glucose and galactose by the reaction
d. One lactase enzyme can catalyze many reactions
When an enzyme catalyzes a reaction:
Select one:
a. Substrate(s) bind in the active site
b. Products bind in the active site
c. The shape of the enzyme remains unchanged
d. The enzyme is consumed by the reaction
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Match the following definitions as they apply to chemical reactions:
|
Catabolic |
· Drag answer here |
|
Anabolic |
· Drag answer here |
|
Endergonic |
· Drag answer here |
|
Exergonic |
· Drag answer here |
· large molecules broken down into smaller ones
· energy exiting the reaction; reactants contain more
· small molecules built into larger ones
· energy entering the reaction; products contain more
q
Is the action of the enzyme illustrated in the video:
Anabolic or catabolic?
Endergonic or exergonic?
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Is the action of lactase:
1. Anabolic or catabolic?
2. Endergonic or exergonic?
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Why is enzyme activity similar to, but not exactly like, a “Lock” and “Key”?
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# Product Molecules/minute at:
|
Amount of Substrate (Lactose) |
pH 3 |
pH 5 |
pH 7 |
pH 9 |
pH 11 |
|
0.5 g |
|||||
|
1.0g
|
|||||
|
2.0g
|
|||||
|
4.0g
|
|||||
|
8.0 g
|
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What substrate amount was required to achieve the maximum reaction rate?
Select one:
a. 1.0 g
b. 4.0 g
c. 2.0 g
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At what pH level did the maximum reaction rate occur?
Select one:
a. 3
b. 1
c. 7
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Why was there no increase in the reaction rate with 8.0 g. of substrate as compared to 4.0 g. of substrate? What would you need to add to see an increase in the reaction rate with 8.0 g. of substrate?
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In the graph you created in the lab simulation with your data:
a. What is represented by the green line?
b. What is the optimal pH for lactase enzyme activity?
